A simple time-resolved fluorescence assay for quantitative determination of DOTA chelator

Anal Biochem. 2019 Nov 1:584:113384. doi: 10.1016/j.ab.2019.113384. Epub 2019 Jul 26.

Abstract

DOTA (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate) is one of the preeminent metal chelator applied for diagnostic and therapeutic purposes, however to date there is no versatile and reliable nonradioisotopic method for its precise determination. In this technical note, we present a novel and sensitive fluorimetric assay for quantitative determination of DOTA based on the luminescence quenching of the highly luminescent europium ions complex with trioctyl phosphine oxide and naphthoyl trifluoroacetone sensitizing activators. The assay is carried out in two simple steps and enables the determination of DOTA in the nanomolar range providing a superior tool compared to commonly applied spectrophotometric assay with Arsenazo-III reagent.

Keywords: Conjugate; DOTA chelator; Europium; Luminescence; Metal complexes; Time-resolved fluorescence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calibration
  • Chelating Agents / analysis*
  • Chelating Agents / chemistry*
  • Heterocyclic Compounds, 1-Ring / chemistry*
  • Spectrometry, Fluorescence / methods*
  • Time Factors

Substances

  • Chelating Agents
  • Heterocyclic Compounds, 1-Ring
  • 1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid