Personalized precision therapy and rapid osseointegration are the main development directions of dental implants. Three-dimensional (3D) printing is the most promising method to fabricate personalized implants with complex design and structure. Rapid osseointegration guarantees the survival of implants especially at early implant time, and the surface modification via nano-technology is considered as the most effective method to promote osseointegration. Herein, the Ti6Al4V implants were fabricated by 3D-printing method then the nano-topographic surface was constructed on them to improve the biological responses. The results showed that the nano-topographic modification favored the adhesion and osteogenic differentiation of bone marrow derived mesenchymal stromal cells (BMSCs), accelerating the rapid osseointegration in vivo in rat condyle of femur model. It is reasonable to predict that 3D-printed implants with nano-topographic surface modification have a promising future in orthopedic applications.