Generation of two induced pluripotent stem cell lines NCCSi005A and NCCSi006A from CD4+T cells of healthy individuals of Indian origin

Mohsina Khan; Samruddhi Zende; Arun Vaidyanath; Rasika Avatade; Anjali Shiras

doi:10.1016/j.scr.2019.101506

Generation of two induced pluripotent stem cell lines NCCSi005A and NCCSi006A from CD4⁺T cells of healthy individuals of Indian origin

Stem Cell Res. 2019 Aug:39:101506. doi: 10.1016/j.scr.2019.101506. Epub 2019 Jul 18.

Authors

Mohsina Khan¹, Samruddhi Zende¹, Arun Vaidyanath¹, Rasika Avatade¹, Anjali Shiras²

Affiliations

¹ National Centre for Cell Science (NCCS), Pune 411007, India.
² National Centre for Cell Science (NCCS), Pune 411007, India. Electronic address: anjali@nccs.res.in.

PMID: 31352200
DOI: 10.1016/j.scr.2019.101506

Abstract

Human induced pluripotent stem cell-lines (iPSCs) of Indian origin NCCSi005A and NCCSi006A were established by reprogramming of CD4⁺T cells, isolated from the peripheral blood mononuclear cells (PBMCs) of two healthy female donors. Reprogramming was achieved using integration free, Sendai viral vector system expressing cocktail of transcription factors KOS, hc-MYC and hKLF4. Both the established cell-lines showed alkaline phosphatase activity, expressed stemness markers, exhibited normal female karyotype and displayed potential for tri-lineage differentiation. These two CD4⁺T cells derived cell-lines represent valuable resource as control iPSC cell lines of Indian origin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CD4-Positive T-Lymphocytes / cytology*
CD4-Positive T-Lymphocytes / metabolism
Cell Line
Cells, Cultured
Embryoid Bodies / cytology
Embryoid Bodies / metabolism
Female
Fluorescent Antibody Technique
Humans
Immunotherapy, Adoptive
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / metabolism
Karyotyping
Microsatellite Repeats / genetics
Receptors, Chimeric Antigen / metabolism

Substances

Receptors, Chimeric Antigen