A rapid and sensitive fluorescent probe for detecting hydrogen polysulfides in living cells and zebra fish

Qian Fang; Xiuxiu Yue; Shaohui Han; Benhua Wang; Xiangzhi Song

doi:10.1016/j.saa.2019.117410

A rapid and sensitive fluorescent probe for detecting hydrogen polysulfides in living cells and zebra fish

Spectrochim Acta A Mol Biomol Spectrosc. 2020 Jan 5:224:117410. doi: 10.1016/j.saa.2019.117410. Epub 2019 Jul 22.

Authors

Qian Fang¹, Xiuxiu Yue¹, Shaohui Han¹, Benhua Wang², Xiangzhi Song³

Affiliations

¹ College of Chemistry & Chemical Engineering, Central South University, Changsha 410083, Hunan Province, China.
² College of Chemistry & Chemical Engineering, Central South University, Changsha 410083, Hunan Province, China. Electronic address: benhuawang@csu.edu.cn.
³ College of Chemistry & Chemical Engineering, Central South University, Changsha 410083, Hunan Province, China; Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, Changsha 410083, Hunan Province, China. Electronic address: xzsong@csu.edu.cn.

PMID: 31352139
DOI: 10.1016/j.saa.2019.117410

Abstract

Hydrogen polysulfides (H₂S_n, n>1) plays crucial roles in many biological processes, while it remains a challenge for rapid and selective detection of H₂S_n. We designed and synthesized a turn-on fluorescent probe (JCCF) for detecting H₂S_n based on a new julolidine-coumarinocoumarin scaffold. H₂S_n could trigger a dramatic fluorescence enhancement (52-fold) with a fast response time and a low detection limit of 98.3 nM (S/N = 3). Moreover, JCCF was successfully applied to image H₂S_n in living cells and zebra fish with low cytotoxicity.

Keywords: Fluorescent probe; Hydrogen polysulfides (H(2)S(n), n>1); Julolidine-coumarinocoumarin; Ultrafast; Zebra fish.

MeSH terms

Animals
Fluorescent Dyes / analysis
Fluorescent Dyes / chemistry*
Humans
Hydrogen Sulfide / analysis*
Limit of Detection
MCF-7 Cells
Microscopy, Confocal / methods
Spectrometry, Fluorescence / methods
Sulfides / analysis*
Zebrafish

Substances

Fluorescent Dyes
Sulfides
polysulfide
Hydrogen Sulfide