Intake of ergosterol increases the vitamin D concentrations in serum and liver of mice

Abstract

Factors that can modify the bioavailability of orally administered vitamin D are not yet widely known. Ergosterol is a common fungal sterol found in food which has a chemical structure comparable to that of vitamin D. This study aimed to investigate the effect of ergosterol on vitamin D metabolism. Therefore, 36 male wild type-mice were randomly subdivided into three groups (n = 12) and received a diet containing 25 μg vitamin D₃ and either 0 mg (control), 2 mg or 7 mg ergosterol per kg diet for 6 weeks. To elucidate the impact of ergosterol on hepatic hydroxylation of vitamin D, human hepatoma cells (HepG2) were treated with different concentrations of ergosterol. Concentrations of vitamin D₃ and 25-hydroxyvitamin D₃ (25(OH)D₃) in cells, livers and kidneys of mice and additionally 24,25-dihydroxyvitamin D₃ (24,25(OH)₂D₃) in serum were quantified by LC-MS/MS. The concentration of 1,25-dihydroxyvitamin D₃ (1,25(OH)₂D₃) in serum was analyzed by commercially-available enzyme immuno assay. The concentrations of cholesterol and triglycerides were analyzed in livers of mice by photometric assays. Analyses revealed that mice receiving 7 mg/kg ergosterol with their diet had 1.3-, 1.7- and 1.5-times higher concentrations of vitamin D₃ in serum, liver and kidney, respectively, than control mice (P < 0.05), whereas no significant effects were observed in mice fed 2 mg/kg ergosterol. The hydroxylation of vitamin D remained unaffected by dietary ergosterol, since the concentration of 25-hydroxyvitamin D₃ in serum and tissues and the concentrations of 1,25(OH)₂D₃ and 24,25(OH)₂D₃ in serum were not different between the three groups of mice. The lipid concentrations in liver were also not affected by dietary ergosterol. Data from the cell culture studies showed that ergosterol did not influence the conversion of vitamin D₃ to 25(OH)D₃. To conclude, ergosterol appears to be a modulator of vitamin D₃ concentrations in the body of mice, without modulating the hydroxylation of vitamin D₃ in liver.

Keywords: Dietary ergosterol; Human hepatoma cells; Mice; Serum; Tissue; Vitamin D metabolites.