Analysis of venom sac constituents from the solitary, aculeate wasp Cerceris rybyensis

Sachin Kote; Jakub Faktor; Irena Dapic; Marcos Yebenes Mayordomo; Mikołaj Kocikowski; Alexander Kagansky; David Goodlett; Borivoj Vojtesek; Ted Hupp; David Wilcockson; Ross Piper

doi:10.1016/j.toxicon.2019.07.012

Analysis of venom sac constituents from the solitary, aculeate wasp Cerceris rybyensis

Toxicon. 2019 Nov:169:1-4. doi: 10.1016/j.toxicon.2019.07.012. Epub 2019 Jul 25.

Authors

Affiliations

¹ University of Gdansk, International Centre for Cancer Vaccine Science, ul. Wita Stwosza 63, 80-308 Gdansk, Poland.
² Regional Centre for Applied Molecular Oncology, Masaryk Memorial Cancer Institute, 656 53 Brno, Czech Republic.
³ University of Gdansk, International Centre for Cancer Vaccine Science, ul. Wita Stwosza 63, 80-308 Gdansk, Poland; University of Edinburgh, Institute of Genetics and Molecular Medicine, Edinburgh, Scotland, EH4 2XR, UK.
⁴ Centre for Genomic and Regenerative Medicine, School of Biomedicine, Far Eastern Federal University, Vladivostok, Russian Federation.
⁵ University of Gdansk, International Centre for Cancer Vaccine Science, ul. Wita Stwosza 63, 80-308 Gdansk, Poland; Department of Pharmaceutical Sciences, University of Maryland, Baltimore, MD, USA; Department of Microbial Pathogenesis, University of Maryland, Baltimore, MD, USA.
⁶ University of Gdansk, International Centre for Cancer Vaccine Science, ul. Wita Stwosza 63, 80-308 Gdansk, Poland; Regional Centre for Applied Molecular Oncology, Masaryk Memorial Cancer Institute, 656 53 Brno, Czech Republic; University of Edinburgh, Institute of Genetics and Molecular Medicine, Edinburgh, Scotland, EH4 2XR, UK.
⁷ IBERS, Aberystwyth University, Aberystwyth, SY23 3FL, UK.
⁸ School of Biology, University of Leeds, Leeds, LS2 9JT, UK. Electronic address: ross_piper@yahoo.com.

PMID: 31352010
DOI: 10.1016/j.toxicon.2019.07.012

Abstract

Solitary aculeate wasps are abundant and diverse hymenopteran insects that disable prey using venom. The venom may possess neuromodulation, immunomodulatory, metabolic-modulatory and antimicrobial functions. Venom analysis of transcriptomes and proteomes has been previously performed in social and parasitoid wasp species. We develop methodologies including mass spectrometry-based shotgun proteomics to analyse the protein constituents from venom sacs of the solitary aculeate wasp Cerceris rybyensis. The venom sac constituents of C. rybyensis are discussed with respect to other wasp species.

Keywords: Cerceris; Hymenoptera; Mass spectrometry; Proteomics; Venom sac.

MeSH terms

Animals
Female
Proteomics
Tandem Mass Spectrometry
Wasp Venoms / chemistry*
Wasps / chemistry*

Substances

Wasp Venoms