First report of the prevalence and genotype of Trypanosoma spp. in bats in Yunnan Province, Southwestern China

Abstract

Trypanosome is considered as one of important parasites in almost all mammalian species, which occurs in Chiroptera throughout the world. Although numerous trypanosome species have been identified in bats in Asia, Africa, South America and Europe, little is known about the genetic diversity and pathogenicity of trypanosomes in Chinese bat. Recently, some human Trypanosoma cruzi infection attributed to a bat-related T. cruzi (TcBat) from the Noctilio spp., Myotis spp. and Artibeus spp was found. Consequently, it is a necessity to know trypanosome species in bats from China. In order to determine the prevalence and genotypes in bat from southwestern China, wehere detected trypanosomes prevalence 227bat brain tissue samples, including 60 Rousettus leschenaultia, 58 Hipposideros Pomona, 69 Rhinolophus pusillus, 40 Myotis daubentonni in Yunnan Province of China using nested PCR based on 18S rRNA. 14 (6.2%) of them were trypanosmes positive including 13 insect-eating bats and 1 fruit bat. The prevalence of trypanosome in R.leschenaultia, H. Pomona, and R.pusillus was 1.67%(1/60), 6.90%(4/58) and 13.0%(9/69), respectively (P < 0.01), suggesting R. pusillus was a main-vector host bat. The positive rate of T.sp, T. dionisii, T.brucei brucei and T.sp ZY-2 was 4.8% (11/227), 0.4%(1/227), 0.4%(1/227), and 0.4% (1/227), resepectively. These results showed that T.sp-Yunnan is the predominant genospecies. To our knowledge, this is the first report about Trypanosome species in bats in Yunnan Province, southwestern China.

Keywords: 18S rRNA; Bats; China; Trypanosome.