Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) for Quantitative Proteomics

Esthelle Hoedt; Guoan Zhang; Thomas A Neubert

doi:10.1007/978-3-030-15950-4_31

Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) for Quantitative Proteomics

Adv Exp Med Biol. 2019:1140:531-539. doi: 10.1007/978-3-030-15950-4_31.

Authors

Esthelle Hoedt¹, Guoan Zhang², Thomas A Neubert³

Affiliations

¹ Kimmel Center for Biology and Medicine at the Skirball Institute and Department of Cell Biology, New York University School of Medicine, New York, NY, USA.
² Proteomics and Metabolomics Core Facility, Weill Cornell Medicine, New York, NY, USA.
³ Kimmel Center for Biology and Medicine at the Skirball Institute and Department of Cell Biology, New York University School of Medicine, New York, NY, USA. Thomas.Neubert@med.nyu.edu.

PMID: 31347069
DOI: 10.1007/978-3-030-15950-4_31

Abstract

Stable isotope labeling by amino acids in cell culture (SILAC) is a powerful approach for high-throughput quantitative proteomics. SILAC allows highly accurate protein quantitation through metabolic encoding of whole cell proteomes using stable isotope labeled amino acids. Since its introduction in 2002, SILAC has become increasingly popular. In this chapter we review the methodology and application of SILAC, with an emphasis on three research areas: dynamics of posttranslational modifications, protein-protein interactions, and protein turnover.

Keywords: Mass spectrometry; Metabolic labeling; Quantitative proteomics; Stable isotope labeling by amino acids in cell culture (SILAC).

Publication types

Review

MeSH terms

Amino Acids / chemistry*
Cell Culture Techniques*
Isotope Labeling*
Proteome
Proteomics / methods*

Substances

Amino Acids
Proteome