A rice gene that confers broad-spectrum resistance to β-triketone herbicides

Science. 2019 Jul 26;365(6451):393-396. doi: 10.1126/science.aax0379.

Abstract

The genetic variation of rice cultivars provides a resource for further varietal improvement through breeding. Some rice varieties are sensitive to benzobicyclon (BBC), a β-triketone herbicide that inhibits 4-hydroxyphenylpyruvate dioxygenase (HPPD). Here we identify a rice gene, HIS1 (HPPD INHIBITOR SENSITIVE 1), that confers resistance to BBC and other β-triketone herbicides. We show that HIS1 encodes an Fe(II)/2-oxoglutarate-dependent oxygenase that detoxifies β-triketone herbicides by catalyzing their hydroxylation. Genealogy analysis revealed that BBC-sensitive rice variants inherited a dysfunctional his1 allele from an indica rice variety. Forced expression of HIS1 in Arabidopsis conferred resistance not only to BBC but also to four additional β-triketone herbicides. HIS1 may prove useful for breeding herbicide-resistant crops.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 4-Hydroxyphenylpyruvate Dioxygenase / antagonists & inhibitors
  • Bridged Bicyclo Compounds / chemistry*
  • Bridged Bicyclo Compounds / pharmacology*
  • Genes, Plant*
  • Herbicide Resistance / genetics*
  • Ketones / chemistry
  • Oryza / drug effects*
  • Oryza / genetics*
  • Oxygenases / genetics*
  • Sulfones / chemistry*
  • Sulfones / pharmacology*

Substances

  • Bridged Bicyclo Compounds
  • Ketones
  • Sulfones
  • benzobicyclon
  • Oxygenases
  • 4-Hydroxyphenylpyruvate Dioxygenase