In recent years, nanoparticle tracking analysis (NTA) has emerged as an alternative tool for particle size characterization. Especially when examining polydisperse systems, individual particle to particle tracking allows for higher peak resolution than dynamic light scattering techniques. However, NTA requires an experienced user with a good insight into how the different settings can affect the determination of particle size and size distributions. This chapter provides a guideline for protein aggregation studies using the example of temperature-induced aggregation of IgG at low concentration.
Keywords: Nanoparticle tracking analysis; Particle visualization; Polydisperse samples; Protein aggregation; Size distribution.