KMT2C modulates migration and invasion processes in osteosarcoma cell lines

Pathol Res Pract. 2019 Oct;215(10):152534. doi: 10.1016/j.prp.2019.152534. Epub 2019 Jul 12.

Abstract

In this study we investigated the role of KMT2C (a chromatin-modifying and remodelling protein) in osteosarcoma progression through cell migration and invasion assays in osteosarcoma primary and metastatic cell lines. Wound healing and transwell assays were used to detect changes of cell migration and matrigel assay was used to evaluate changes of cell invasion in primary and metastatic osteosarcoma cell lines after KMT2C siRNA transfection. We found that primary osteosarcoma cell lines showed the highest capacity of migration before mRNA KMT2C silencing and the highest capacity of invasion after mRNA KMT2C silencing; on the contrary, osteosarcoma metastatic cell line showed the highest capacity of migration after mRNA KMT2C silencing and the highest capacity of invasion before mRNA KMT2C silencing. Our study supports data in favour of selective enhancer changes, KMT2C-mediated, in metastatic osteosarcoma probably due to the different microenvironment between primary and metastatic sites.

Keywords: Invasion; KMT2C; Metastasis; Migration; Osteosarcoma.

MeSH terms

  • Bone Neoplasms / metabolism*
  • Bone Neoplasms / pathology
  • Cell Line, Tumor
  • Cell Movement / physiology*
  • Cell Proliferation / physiology
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Humans
  • Neoplasm Invasiveness / pathology*
  • Osteosarcoma / metabolism*
  • Osteosarcoma / pathology
  • RNA, Small Interfering
  • Wound Healing / physiology

Substances

  • DNA-Binding Proteins
  • KMT2C protein, human
  • RNA, Small Interfering