SpyTag-SpyCatcher Chemistry for Protein Bioconjugation In Vitro and Protein Topology Engineering In Vivo

Xiao-Wei Wang; Wen-Bin Zhang

doi:10.1007/978-1-4939-9654-4_19

SpyTag-SpyCatcher Chemistry for Protein Bioconjugation In Vitro and Protein Topology Engineering In Vivo

Methods Mol Biol. 2019:2033:287-300. doi: 10.1007/978-1-4939-9654-4_19.

Authors

Xiao-Wei Wang¹, Wen-Bin Zhang²

Affiliations

¹ Beijing National Laboratory for Molecular Sciences, Key Laboratory of Polymer Chemistry and Physics of Ministry of Education, Center for Soft Matter Science and Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing, People's Republic of China.
² Beijing National Laboratory for Molecular Sciences, Key Laboratory of Polymer Chemistry and Physics of Ministry of Education, Center for Soft Matter Science and Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing, People's Republic of China. wenbin@pku.edu.cn.

PMID: 31332761
DOI: 10.1007/978-1-4939-9654-4_19

Abstract

The emergence of "molecular superglue," such as SpyTag-SpyCatcher chemistry, has tremendously expanded our capability in manipulating protein shape and architecture via conjugation. Telechelic proteins bearing the SpyTag and SpyCatcher reactive sequences can be expressed and purified for bioconjugation in vitro, giving protein conjugates, branched proteins, and circular proteins. By encoding both reactive sequences in the same construct for expression in vivo, the nascent protein undergoes programmed posttranslational modification guided by protein folding and reaction, leading to diverse nonlinear topologies in situ. In this chapter, we present the SpyTag-SpyCatcher chemistry as a versatile platform for protein bioconjugation and topology engineering.

Keywords: Protein bioconjugation; SpyCatcher; SpyTag; Topology Engineering.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Expression / genetics
Protein Engineering / methods*
Proteins / genetics*

Substances

Proteins