Transcription factor HBP1: A regulator of senescence and apoptosis of preadipocytes

Hongyan Chen; Chang Liu; Yumeng Liu; Hui Li; Bohan Cheng

doi:10.1016/j.bbrc.2019.07.048

Transcription factor HBP1: A regulator of senescence and apoptosis of preadipocytes

Biochem Biophys Res Commun. 2019 Sep 17;517(2):216-220. doi: 10.1016/j.bbrc.2019.07.048. Epub 2019 Jul 20.

Authors

Hongyan Chen¹, Chang Liu¹, Yumeng Liu¹, Hui Li¹, Bohan Cheng²

Affiliations

¹ Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, 150030, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin, 150030, Heilongjiang, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, Heilongjiang, China.
² Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, 150030, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin, 150030, Heilongjiang, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, Heilongjiang, China. Electronic address: chengbohan1027@126.com.

PMID: 31331641
DOI: 10.1016/j.bbrc.2019.07.048

Abstract

Background: /aim: HMG-box protein 1 (HBP1) plays an important role in the senescence and apoptosis of mammalian cells, but its role in chicken cells remains unclear. The aim of this study was to investigate the effects of HBP1 on senescence and apoptosis of chicken preadipocytes.

Methods: The immortalized chicken preadipocyte cell line (ICP2) was used as a cell model. Chicken HBP1 knockout and overexpressing preadipocyte cell lines were established using CRISPR/Cas9 gene editing technology and lentiviral infection. Western blotting was used to detect the protein expression of HBP1 and senescence markers p16 and p53. Cell senescence was measured by Sa-β-Gal staining and apoptosis was detected by flow cytometry.

Results: HBP1 was highly expressed in senescent ICP2 cells compared with young ICP2 cells. After the deletion of HBP1, the degree of senescence, the apoptosis rate and the protein expression levels of p16 and p53 were significantly reduced. After the overexpression of HBP1, the degree of senescence, the apoptosis rate and the protein expression levels of p16 and p53 were significantly increased.

Conclusion: HBP1 promotes the senescence and apoptosis of chicken preadipocytes.

Keywords: Apoptosis; Chicken; HBP1; Preadipocytes; Senescence.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adipocytes / cytology*
Adipocytes / metabolism
Animals
Apoptosis*
Avian Proteins / genetics
Avian Proteins / metabolism*
Cell Line
Cellular Senescence
Chickens / genetics
Chickens / metabolism*
Gene Deletion
High Mobility Group Proteins / genetics
High Mobility Group Proteins / metabolism*
Transcription Factors / genetics
Transcription Factors / metabolism
Up-Regulation

Substances

Avian Proteins
High Mobility Group Proteins
Transcription Factors