This research shows that high-performance biosensors can be produced by modification of screen-printed electrodes with enzymes and conducting hydrogel based on sol-gel matrix and single-walled carbon nanotubes. Tetraethoxysilane, dimethyldiethoxysilane and polyvinyl alcohol were used as the sol-gel matrix basis. Modified SWCNT provide direct electron transfer during glucose oxidation, as confirmed by cyclic voltammetry. The developed conducting sol-gel screen-printed electrodes can determine glucose within the concentration range 0.045-1.04 mM. The developed biosensor is not only in pace with its world analogues but even exceeds them by some analytical and metrological properties. The developed conducting sol-gel biosensor was used to measure the concentration of glucose in blood. The test results differed only insignificantly from those received with the help of standard glucose meter.
Keywords: Amperometric glucose biosensor; Biocatalyst; ORMOSIL; Organic–inorganic hybrid sol–gel.