Performance of various cyanide degrading bacteria on the biodegradation of free cyanide in water

L C Razanamahandry; C T Onwordi; W Saban; A K H Bashir; L Mekuto; E Malenga; E Manikandan; E Fosso-Kankeu; M Maaza; S K O Ntwampe

doi:10.1016/j.jhazmat.2019.120900

Performance of various cyanide degrading bacteria on the biodegradation of free cyanide in water

J Hazard Mater. 2019 Dec 15:380:120900. doi: 10.1016/j.jhazmat.2019.120900. Epub 2019 Jul 15.

Authors

L C Razanamahandry¹, C T Onwordi², W Saban³, A K H Bashir³, L Mekuto⁴, E Malenga⁵, E Manikandan⁶, E Fosso-Kankeu⁵, M Maaza³, S K O Ntwampe⁷

Affiliations

¹ UNESCO UNISA Africa Chair in Nanoscience's/Nanotechnology Laboratories (U2AC2N), College of Graduate Studies, University of South Africa (UNISA), Muckleneuk Ridge, P.O. Box 392, Pretoria, South Africa; Nanosciences African network (NANOAFNET), Materials Research Group (MRG), iThemba LABS-National Research Foundation (NRF), 1 Old Faure Road, 7129, P.O. Box 722, Somerset West, Western Cape Province, Cape Town, South Africa. Electronic address: clrazanamahandry@tlabs.ac.za.
² University of Western Cape, Environmental and Nano Sciences, Department of Chemistry, Faculty of Natural Sciences, Bellville, Private mail Bag X17, Cape Town, 7535, South Africa.
³ UNESCO UNISA Africa Chair in Nanoscience's/Nanotechnology Laboratories (U2AC2N), College of Graduate Studies, University of South Africa (UNISA), Muckleneuk Ridge, P.O. Box 392, Pretoria, South Africa; Nanosciences African network (NANOAFNET), Materials Research Group (MRG), iThemba LABS-National Research Foundation (NRF), 1 Old Faure Road, 7129, P.O. Box 722, Somerset West, Western Cape Province, Cape Town, South Africa.
⁴ University of Johannesburg, Department of Chemical Engineering, Johannesburg, South Africa.
⁵ Water Pollution Monitoring and Remediation Initiatives Research Group, School of Chemical and Minerals Engineering, North-West University, Private Bag X1290, Potchefstroom, 2520, South Africa.
⁶ UNESCO UNISA Africa Chair in Nanoscience's/Nanotechnology Laboratories (U2AC2N), College of Graduate Studies, University of South Africa (UNISA), Muckleneuk Ridge, P.O. Box 392, Pretoria, South Africa; Nanosciences African network (NANOAFNET), Materials Research Group (MRG), iThemba LABS-National Research Foundation (NRF), 1 Old Faure Road, 7129, P.O. Box 722, Somerset West, Western Cape Province, Cape Town, South Africa; Thiruvalluvar University, Department of Physics, TUCAS Campus, Thennangur, 604408, Vellore, India.
⁷ Bioresource Engineering Research Group (BioERG), Cape Peninsula University of Technology, P.O. Box 652, Cape Town 8000, South Africa; Department of Chemical Engineering, Faculty of Engineering and the Built Environment, Cape Peninsula University of Technology, PO Box 1906, Bellville, 7535, Cape Town, South Africa.

PMID: 31326841
DOI: 10.1016/j.jhazmat.2019.120900

Abstract

This study reports on the biodegradation of free cyanide (FCN) by cyanide degrading bacteria (CDB) that were isolated from mining wastewater and thiocyanate containing wastewater. The performance of these isolates was compared to cryopreserved CDBs that were used in previous studies. The performance of the isolates to degrade FCN was studied in batch cultures. It was observed that the CDB from the thiocyanate wastewater showed higher biodegradation rates (2.114 g CN^-. L^-1.O.D_{600 nm}^-1.h^-1) compared to the isolates from the mining wastewater. The isolates from the cryopreserved CDBs and from the mining wastewater achieved a biodegradation rate of 1.285 g CN^- L^-1.O.D_{600 nm}^-1.h^-1 and 1.209 g CN^-.L^-1.O.D_{600 nm}^-1.h^-1, respectively. This study demonstrated that the source of the organisms plays a significant role on FCN biodegradation.

Keywords: Bioremediation; Cyanide degrading bacteria; Free cyanide; Microorganism; Mining wastewater.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteria / metabolism*
Biodegradation, Environmental*
Cyanides / metabolism*
Mining
Wastewater
Water Pollutants, Chemical / metabolism*

Substances

Cyanides
Waste Water
Water Pollutants, Chemical