Economical production of androstenedione and 9α-hydroxyandrostenedione using untreated cane molasses by recombinant mycobacteria

Xiuling Zhou; Yang Zhang; Yanbing Shen; Xiao Zhang; Zhenjian Zhang; Shuangping Xu; Jianmei Luo; Menglei Xia; Min Wang

doi:10.1016/j.biortech.2019.121750

Economical production of androstenedione and 9α-hydroxyandrostenedione using untreated cane molasses by recombinant mycobacteria

Bioresour Technol. 2019 Oct:290:121750. doi: 10.1016/j.biortech.2019.121750. Epub 2019 Jul 9.

Authors

Xiuling Zhou¹, Yang Zhang², Yanbing Shen³, Xiao Zhang¹, Zhenjian Zhang¹, Shuangping Xu¹, Jianmei Luo¹, Menglei Xia¹, Min Wang⁴

Affiliations

¹ Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.
² Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China; College of Life Science, Liaocheng University, Liaocheng, Shandong 252059, China. Electronic address: zhy@lcu.edu.cn.
³ Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China; Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, 300457 Tianjin, China.
⁴ Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China; Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, 300457 Tianjin, China. Electronic address: minw@tust.edu.cn.

PMID: 31325842
DOI: 10.1016/j.biortech.2019.121750

Abstract

Production of androstenedione (AD) and 9α-hydroxyandrostenedione (9α-OH-AD) by recombinant mycobacteria using untreated cane molasses and hydrolysate of mycobacterial cells (HMC) was investigated for the first time. B-vitamins feeding experiment and reverse transcription-PCR analysis showed that propionyl-CoA carboxylase (PCC) plays an important role in the phytosterol biotransformation of mycobacteria. The respective AD and 9α-OH-AD conversion ratios were increased by 2.91 and 1.48 times through coexpression of PCC and NADH dehydrogenase. The highest conversion ratios of AD and 9α-OH-AD obtained by using a co-feeding strategy of cane molasses and HMC reached 96.38% and 95.04%, respectively, and the total costs of carbon and nitrogen sources for the culture medium were reduced by 29.89% and 49.49%, respectively. Taking the results together, untreated cane molasses and HMC can be used for the economical production of steroidal pharmaceutical precursors by mycobacteria. This study offers an economical and green strategy for steroidal pharmaceutical precursor production.

Keywords: Cane molasses; Hydrolysate of mycobacteria cells; Mycobacteria; Phytosterol biotransformation; Propionyl-CoA carboxylase.

MeSH terms

Androstenedione
Canes
Molasses*
Mycobacterium*
Nitrogen

Substances

Androstenedione
Nitrogen