Isolation and characterization of a chlorate-reducing bacterium Ochrobactrum anthropi XM-1

Han-Wen Chen; Meng Xu; Xi-Wen Ma; Zhong-Hua Tong; Dong-Feng Liu

doi:10.1016/j.jhazmat.2019.120873

Isolation and characterization of a chlorate-reducing bacterium Ochrobactrum anthropi XM-1

J Hazard Mater. 2019 Dec 15:380:120873. doi: 10.1016/j.jhazmat.2019.120873. Epub 2019 Jul 12.

Authors

Han-Wen Chen¹, Meng Xu¹, Xi-Wen Ma¹, Zhong-Hua Tong², Dong-Feng Liu¹

Affiliations

¹ CAS Key Laboratory of Urban Pollutant Conversion, Department of Applied Chemistry, University of Science & Technology of China, Hefei, 230026, China.
² CAS Key Laboratory of Urban Pollutant Conversion, Department of Applied Chemistry, University of Science & Technology of China, Hefei, 230026, China; Anhui Province Key Laboratory of Polar Environment and Global Change, University of Science & Technology of China, Hefei, 230026, China. Electronic address: zhtong@ustc.edu.cn.

PMID: 31325697
DOI: 10.1016/j.jhazmat.2019.120873

Abstract

A Gram-negative chlorate-reducing bacterial strain XM-1 was isolated. The 16S rRNA gene sequence identified the isolate as Ochrobactrum anthropi XM-1, which was the first strain of genus Ochrobactrum reported having the ability to reduce chlorate. The optimum growth temperature and pH for strain XM-1 to reduce chlorate was found to be 30 °C and 5.0-7.5, respectively, under anaerobic condition. Strain XM-1 could tolerate high chlorate concentration (200 mM), and utilize a variety of carbohydrates (glucose, L-arabinose, D-fructose, sucrose), glycerin and sodium citrate as electron donors. In addition, oxygen and nitrate could be used as electron acceptors, but perchlorate could not be reduced. Enzyme activities related to chlorate reducing were characterized in cell extracts. Activities of chlorate reductase and chlorite dismutase could be detected in XM-1 cells grown under both aerobic and anaerobic conditions, implying the two enzymes were constitutively expressed. This work suggests a high potential of applying Ochrobactrum anthropi XM-1 for remediation of chlorate contamination.

Keywords: Chlorate reductase; Chlorate reduction; Chlorate-reducing bacteria; Chlorite dismutase; Ochrobactrum anthropic XM-1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aerobiosis
Anaerobiosis
Chlorates / metabolism*
Hydrogen-Ion Concentration
Ochrobactrum anthropi / growth & development
Ochrobactrum anthropi / isolation & purification*
Ochrobactrum anthropi / metabolism*
Oxidation-Reduction
Oxidoreductases / metabolism

Substances

Chlorates
Oxidoreductases
chlorite dismutase
chlorate reductase