Short Communication: Preliminary Differences Identified in Genes Responsible for Biofilm Formation in Poultry Isolates of Salmonella enterica Heidelberg, Enteritidis, and Kentucky

Zhaohao Shi; Dana K Dittoe; Kristina M Feye; Mike Kogut; Steven C Ricke

doi:10.3390/microorganisms7070196

Short Communication: Preliminary Differences Identified in Genes Responsible for Biofilm Formation in Poultry Isolates of Salmonella enterica Heidelberg, Enteritidis, and Kentucky

Microorganisms. 2019 Jul 9;7(7):196. doi: 10.3390/microorganisms7070196.

Authors

Zhaohao Shi¹, Dana K Dittoe¹, Kristina M Feye², Mike Kogut², Steven C Ricke³

Affiliations

¹ Center for Food Safety and Food Science Department, University of Arkansas, Fayetteville, AR 72704, USA.
² Southern Plains Agricultural Research Center, United States Department of Agriculture-Agricultural Research Unit, College Station, TX 77845, USA.
³ Center for Food Safety and Food Science Department, University of Arkansas, Fayetteville, AR 72704, USA. sricke@uark.edu.

Abstract

Salmonella enterica is one of the most prevalent foodborne pathogens. The large quantity of serovar types results in the colonization of a large spectrum of hosts, with different environmental conditions and hazards. The aim of this study was to evaluate the differences in gene expression (bcsA and csgD) of Salmonella enterica serovars Heidelberg, Kentucky, and Enteritidis during biofilm formation using quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR). Overall, there appeared to be differences in expression between the different serovars with high variation between strains. These data are important as they demonstrate considerable variability in gene expression between serovars and strains of poultry isolates of Salmonella enterica.

Keywords: Salmonella Enteritidis; Salmonella Heidelberg; Salmonella Kentucky; biofilm; pellicle; qRT-PCR.