Role of Myeloperoxidase of northern snakehead (Channa argus) in Aeromonas veronii infection

Chong Chen; Bintong Yang; Sayed Haidar Abbas Raza; Dongxing Zhang; Tonglei Wu; Zhiqiang Zhang; Irfan Ullah; Rajwali Khan; Guilian Yang; Chunfeng Wang; Guiqin Wang; Xiaofeng Shan; Yuanhuan Kang; Aidong Qian

doi:10.1016/j.micpath.2019.103622

Role of Myeloperoxidase of northern snakehead (Channa argus) in Aeromonas veronii infection

Microb Pathog. 2019 Oct:135:103622. doi: 10.1016/j.micpath.2019.103622. Epub 2019 Jul 17.

Authors

Affiliations

¹ College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, Jilin, 130118, China.
² College of Animal Science and Technology, Northwest A&F University, Yangling, Shanxi, 712100, PR China. Electronic address: haiderraza110@nwafu.edu.cn.
³ College of Animal Science and Technology, Key Laboratory of Preventive Veterinary Medicine, Hebei Normal University of Science and Technology, Changli, Hebei, 066600, China.
⁴ Department of Zoology, Hazara University, Mansehra Khyber Pakhtunkhwa, 21300, Pakistan.
⁵ College of Animal Science and Technology, Northwest A&F University, Yangling, Shanxi, 712100, PR China.
⁶ College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, Jilin, 130118, China. Electronic address: kangyuanhuan@jlau.edu.cn.
⁷ College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, Jilin, 130118, China. Electronic address: qianaidong0115@163.com.

PMID: 31323322
DOI: 10.1016/j.micpath.2019.103622

Abstract

Myeloperoxidase (MPO) is a ferrous lysosomal protein with many immune functions that belongs to the heme peroxidase enzyme. In this study, the functions of MPO in the northern snakehead (Channa argus) were investigated by cloning an MPO cDNA sequence with a full length of 3181 bp. Homology analysis showed that northern snakehead MPO gene had the highest (81%) homology with mandarin fish (Siniperca chuatsi). In healthy northern snakehead, the MPO gene was expressed in the head-kidney, kidney, heart, gill, spleen, liver, and muscles but not midgut. After the northern snakehead was infected with Aeromonas veronii, the MPO gene expression varied in different tissues with low level in spleen, liver, gill and muscle, fluctuated in kidney and head-kidney and showed high level in heart. The result indicated that MPO might play an important role in the antimicrobial immune response of the northern snakehead.

Keywords: Aeromonas veronii; Expression analysis; MPO gene; Northern snakehead (Channa argus).

MeSH terms

Aeromonas veronii / pathogenicity*
Animals
Base Sequence
Cloning, Molecular
DNA, Complementary
Fish Diseases / microbiology*
Fishes / genetics
Fishes / metabolism*
Gene Expression
Gram-Negative Bacterial Infections / veterinary*
Heart
Kidney / pathology
Liver / metabolism
Muscles / metabolism
Peroxidase / classification
Peroxidase / genetics
Peroxidase / physiology*
Phylogeny
Spleen / metabolism

Substances

DNA, Complementary
Peroxidase