Structure, rheology, and copper-complexation of a hyaluronan-like exopolysaccharide from Vibrio

Manuel Martin-Pastor; Andreia S Ferreira; Xavier Moppert; Cláudia Nunes; Manuel A Coimbra; Rui L Reis; Jean Guezennec; Ramon Novoa-Carballal

doi:10.1016/j.carbpol.2019.114999

Structure, rheology, and copper-complexation of a hyaluronan-like exopolysaccharide from Vibrio

Carbohydr Polym. 2019 Oct 15:222:114999. doi: 10.1016/j.carbpol.2019.114999. Epub 2019 Jun 18.

Authors

Manuel Martin-Pastor¹, Andreia S Ferreira², Xavier Moppert³, Cláudia Nunes⁴, Manuel A Coimbra², Rui L Reis⁵, Jean Guezennec⁶, Ramon Novoa-Carballal⁷

Affiliations

¹ Unidade de Resonancia Magnética, RIAIDT, CACTUS, Campus Vida, Univ. Santiago de Compostela, 15782, A Coruña, Spain.
² QOPNA/LAQV, Departamento de Química, Universidade de Aveiro, Campus de Santiago, 3810-193, Aveiro, Portugal.
³ Pacific Biotech BP 140 289 Arue Tahiti (Polynésie Française), France.
⁴ CICECO, Departamento de Química, Universidade de Aveiro, Campus de Santiago, 3810-193, Aveiro, Portugal.
⁵ 3B´s Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal; ICVS/3B's - PT Government Associate Laboratory, Braga, Guimarães, Portugal; The Discoveries Centre for Regenerative and Precision Medicine, Headquarters at University of Minho, Avepark, 4805-017 Barco, Guimarães, Portugal.
⁶ Pacific Biotech BP 140 289 Arue Tahiti (Polynésie Française), France. Electronic address: guezennec.jean@wanadoo.fr.
⁷ 3B´s Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal; ICVS/3B's - PT Government Associate Laboratory, Braga, Guimarães, Portugal. Electronic address: rnovoausc@yahoo.es.

PMID: 31320058
DOI: 10.1016/j.carbpol.2019.114999

Abstract

MO245 exopolysaccharide (EPS) was produced in laboratory conditions from Vibrio genus microorganism isolated from bacterial mats found in Moorea Island. Its structure consists of a linear tetrasaccharide repeating unit →4)-β-D-GlcpA-(1→4)-α-D-GalpNAc-(1→3)-β-D-GlcpNAc-(1→4)-β-D-GlcpA-(1→ containing covalently-linked 5% of glucose, galactose, and rhamnose, determined by methylation analyses and NMR spectroscopy. The molecular weight, radius of gyration (R_g) and intrinsic viscosity, [η], determined by gel permeation chromatography with light scattering and viscosity detection, were 513 ± 4 kDa (PDI, 1.42 ± 0.01), 6.7 ± 0.3 dl/g and 56 ± 0.3 nm respectively. The chelation of the EPS with copper divalent ions leads to the instantaneous formation of gels. The structural similitude proposed, based in an equal ratio of GlcA to N-acetylated sugars and in the same type of glyosidic linkages present in the repeating unit (alternated 1→3 and 1→4 linkages), is translated into analogous physicochemical properties: MO245 EPS is a flexible polyelectrolyte, with scaling exponents similar to that described for HA. This similitude opens opportunities in future drug delivery, tissue engineering, and cosmetic applications.

Keywords: Bacterial mats; Exopolysaccharide; Hyaluronic-like; NMR spectroscopy; Physicochemical properties; Vibrio sp.