Transcription factor WRKY30 mediates resistance to Cucumber mosaic virus in Arabidopsis

Lijuan Zou; Feng Yang; Yonghong Ma; Qinggui Wu; Kexian Yi; Dawei Zhang

doi:10.1016/j.bbrc.2019.07.030

Transcription factor WRKY30 mediates resistance to Cucumber mosaic virus in Arabidopsis

Biochem Biophys Res Commun. 2019 Sep 10;517(1):118-124. doi: 10.1016/j.bbrc.2019.07.030. Epub 2019 Jul 13.

Authors

Lijuan Zou¹, Feng Yang², Yonghong Ma³, Qinggui Wu¹, Kexian Yi⁴, Dawei Zhang⁵

Affiliations

¹ Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, Sichuan, 637009, China; Ecological Security and Protection Key Laboratory of Sichuan Province and Life Science and Technology College, Mianyang Normal University, Mianyang, Sichuan, 621000, China.
² Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, Sichuan, China; Rice and Sorghum Institute, Sichuan Academy of Agricultural Sciences, Deyang, 618000, Sichuan, China.
³ Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, Sichuan, 637009, China.
⁴ Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, Sichuan, 637009, China; Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, Hainan, China. Electronic address: yikexian@21cn.com.
⁵ Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, Sichuan, 637009, China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, Sichuan, China. Electronic address: zhdawei@scu.edu.cn.

PMID: 31311650
DOI: 10.1016/j.bbrc.2019.07.030

Abstract

WRKY transcription factors are key regulators in regulating abiotic or biotic stress response in Arabidopsis. Previous studies showed that WRKY30 expression was induced by oxidative stress treatment, fungal elicitor, SA and ABA. However, functions of WRKY30 on viral defense are not well studied. Here, we found that Arabidopsis WRKY DNA binding protein 30 (WRKY30) plays essential roles in regulating Cucumber mosaic virus (CMV) resistance. The expression of WRKY30 was induced by CMV infection and wrky30 mutant displayed more susceptibility (including higher oxidative damages, induced reactive oxygen species synthesis and more PSII photochemistry compromise), while WRKY30 overexpression plants (WRKY30OX) exhibited more resistance to CMV infection. Moreover BRs-induced CMV tolerance is partly dependent on WRKY30. And WRKY30 expression increased after BL treatment. All these demonstrated that WRKY30 works as a positive regulator in plant CMV resistance process.

Keywords: Antiviral defense; Brassinosteroid; CMV; Transcription factor; WRKY30.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis / genetics*
Arabidopsis / virology
Arabidopsis Proteins / genetics*
Cucumovirus / physiology*
DNA-Binding Proteins / genetics*
Disease Resistance
Gene Expression Regulation, Plant
Host-Pathogen Interactions
Plant Diseases / genetics*
Plant Diseases / virology
Plants, Genetically Modified / genetics
Plants, Genetically Modified / virology
Transcription Factors / genetics*
Up-Regulation

Substances

AT5G24110 protein, Arabidopsis
Arabidopsis Proteins
DNA-Binding Proteins
Transcription Factors