Assessment of Proteus mirabilis Antigen Immunological Complexes by Atomic Force Microscopy

Methods Mol Biol. 2019:2021:273-283. doi: 10.1007/978-1-4939-9601-8_23.

Abstract

Atomic force microscopy (AFM) is being increasingly used to directly measure protein interactions in nearly physiological environments. Here, protocols for atomic force microscopy (AFM) for visualization of antigen-antibody complexes are presented. The technique is used to demonstrate complexes formed by rheumatoid arthritis patient antibodies with lipopolysaccharide (LPS) isolated from P. mirabilis (O3) strain S1959 and a synthetic antigen (LPS epitope of 6 N-alpha-(D-galacturonoyl)-L-lysine residues).

Keywords: Atomic force microscopy; Lipopolysaccharide; Proteus mirabilis; Rheumatoid arthritis antibodies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Bacterial / metabolism
  • Antigen-Antibody Complex / isolation & purification*
  • Arthritis, Rheumatoid / immunology*
  • Epitopes / immunology
  • Humans
  • Lipopolysaccharides / immunology
  • Microscopy, Atomic Force
  • Proteus mirabilis / metabolism*

Substances

  • Antibodies, Bacterial
  • Antigen-Antibody Complex
  • Epitopes
  • Lipopolysaccharides
  • lipopolysaccharide, Proteus