A new ratiometric fluorescent probe for sensing lysosomal HOCl based on fluorescence resonance energy transfer strategy

Spectrochim Acta A Mol Biomol Spectrosc. 2019 Dec 5:223:117355. doi: 10.1016/j.saa.2019.117355. Epub 2019 Jul 8.

Abstract

In this paper, ratiometric imaging of lysosomal HOCl was realized with a molecular probe (CR-Ly) based on fluorescence resonance energy transfer by using coumarin as the donor and rhodamine as acceptor. CR-Ly showed high sensitivity and fast response to HOCl. Moreover, CR-Ly exhibited excellent selectivity and sensitivity for HOCl over other biologically relevant species. Furthermore, it was successfully utilized to image the endogenous HOCl with low cytotoxity. And CR-Ly was capable of targeting lysosomes and monitoring lysosomal hypochlorous acid changes owing to the presence of the morpholine moiety. We believe that probe CR-Ly would be helpful to further research on the HOCl-associated diseases in lysosomes.

Keywords: FRET; Fluorescent probe; HOCl; Lysosomes; Ratiometric.

MeSH terms

  • Animals
  • Cell Death
  • Fluorescence Resonance Energy Transfer / methods*
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • Hypochlorous Acid / analysis*
  • Hypochlorous Acid / chemistry
  • Lysosomes / metabolism*
  • Mice
  • RAW 264.7 Cells

Substances

  • Fluorescent Dyes
  • Hypochlorous Acid