Generation of a NKX2.1 knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-2)

Ruth Olmer; Julia Dahlmann; Sylvia Merkert; Sandra Baus; Gudrun Göhring; Ulrich Martin

doi:10.1016/j.scr.2019.101492

Generation of a NKX2.1 knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-2)

Stem Cell Res. 2019 Aug:39:101492. doi: 10.1016/j.scr.2019.101492. Epub 2019 Jun 28.

Authors

Ruth Olmer¹, Julia Dahlmann², Sylvia Merkert², Sandra Baus², Gudrun Göhring³, Ulrich Martin²

Affiliations

¹ Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, 30625 Hannover, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, 30625 Hannover, Germany; Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), Member of the German Center for Lung Research (DZL), Germany. Electronic address: olmer.ruth@mh-hannover.de.
² Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, 30625 Hannover, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, 30625 Hannover, Germany; Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), Member of the German Center for Lung Research (DZL), Germany.
³ Department of Human Genetics, Hannover Medical School, 30625 Hannover, Germany.

PMID: 31299562
DOI: 10.1016/j.scr.2019.101492

Abstract

NK homeobox 1 (NKX2.1; also known as thyroid transcription factor 1, TTF-1) encodes for a transcription factor involved in the development of thyroid, lung and brain. Here, we established a NKX2.1 knock-in reporter cell line from human induced pluripotent stem cells (iPSCs) using TALEN technology. The reporter enables the optimization and monitoring of the differentiation of pluripotent stem cells (PSCs) into NKX2.1 expressing cells on a single cell level, as well as the enrichment of NKX2.1 positive cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Differentiation / genetics
Cell Differentiation / physiology
Cell Line
Flow Cytometry
Humans
Immunohistochemistry
Karyotype
Mice
Polymerase Chain Reaction
Thyroid Nuclear Factor 1 / genetics
Thyroid Nuclear Factor 1 / metabolism*

Substances

Thyroid Nuclear Factor 1