Isolation and identification of tiger parvovirus in captive siberian tigers and phylogenetic analysis of VP2 gene

Kai Wang; Shuaishuai Du; Yiqi Wang; Shaoying Wang; Xiaoqing Luo; Yuanyuan Zhang; Cunfa Liu; Haijun Wang; Zhihua Pei; Guixue Hu

doi:10.1016/j.meegid.2019.103957

Isolation and identification of tiger parvovirus in captive siberian tigers and phylogenetic analysis of VP2 gene

Infect Genet Evol. 2019 Nov:75:103957. doi: 10.1016/j.meegid.2019.103957. Epub 2019 Jul 9.

Authors

Affiliations

¹ College of Animal Science and Technology, Jilin Agricultural University, Xincheng Street No. 2888, Changchun, PR China. Electronic address: wk197811@jlau.edu.cn.
² College of Animal Science and Technology, Jilin Agricultural University, Xincheng Street No. 2888, Changchun, PR China.
³ Wildlife Ambulance Breeding Center of Jilin Province, Jingyue Street No.10500, Changchun, PR China.

PMID: 31299323
DOI: 10.1016/j.meegid.2019.103957

Abstract

To better understand the prevalence and molecular epidemiology of parvovirus, this study reports the isolation and characterization of a tiger parvovirus (TPV) named CHJL-Siberian Tiger-01/2017 from a captive Siberian tiger in Jilin Province, China. A phylogenetic tree based on the full-length VP2 nucleotide sequence was constructed using the isolated strain in this study and 56 reference strains. The results showed that all the parvoviruses can be grouped into two large branches: the canine parvovirus (CPV) branch and the feline parvovirus (FPV) branch. FPV strains comprised TPVs, FPVs, blue fox parvoviruses (BFPVs), mink enteritis viruses (MEVs), and raccoon feline parvoviruses (RFPVs), and CPV strains comprised CPVs and raccoon dog parvoviruses (RDPVs). RFPVs are also often very closely related to those sampled from other carnivorous species, and raccoons may represent conduits for parvovirus transmission to other hosts. The results of amino acid changes in the VP2 protein of the isolated strain showed that amino acid Ile 101 was mutated to Thr (I 101T). Taken together, a field TPV strain CHJL-Siberian Tiger-01/2017 was isolated, which may be suitable for future studies on FPV infection, replication and vaccine development. This study provided new important findings about the evolution of parvovirus infection in tigers.

Keywords: Mutation; Parvovirus; Phylogenetic analysis; Siberian tigers.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Capsid Proteins / genetics*
Gene Expression Regulation, Viral
Parvoviridae Infections / veterinary*
Parvoviridae Infections / virology
Parvovirus / genetics*
Parvovirus / isolation & purification*
Tigers / virology*

Substances

Capsid Proteins