Environmental DNA (eDNA) from juvenile jack mackerel Trachurus japonicus was detected in tanks with 1, 3, 10, or 30 individuals per tank. Quantitative PCR using a set of species-specific primers and a probe revealed that the concentration of eDNA increased almost linearly with the density of fish. The coefficient of determination (R2 ) in the linear regression was lower than values previously reported for freshwater fishes in similar settings.
Keywords: carangidae; marine fish biomass estimation; real-time quantitative PCR; species-specific primers and probe.
© 2019 The Fisheries Society of the British Isles.