Intrinsic role of coagulase negative staphylococci norA-like efflux system in fluoroquinolones resistance

Ligozzi Marco; Galia Liliana; Bertoncelli Anna; Mazzariol Annarita

doi:10.3934/microbiol.2017.4.908

Intrinsic role of coagulase negative staphylococci norA-like efflux system in fluoroquinolones resistance

AIMS Microbiol. 2017 Nov 14;3(4):908-914. doi: 10.3934/microbiol.2017.4.908. eCollection 2017.

Authors

Ligozzi Marco¹, Galia Liliana¹, Bertoncelli Anna¹, Mazzariol Annarita¹

Affiliation

¹ Department of Diagnostics and Public Health, University of Verona, Verona, Italy.

Abstract

NorA is a Staphylococcus aureus multidrug transporter that exports structurally distinct compounds including fluoroquinolones. In this study norA-like genes of Staphylococcus epidermidis (norA _SEP) and Staphylococcus haemolyticus (norA _SHAE) were identified and sequenced. The nucleotide identity of norA _SEP and norA _SHAE with norA was 75.3 and 74.1%, respectively, and the amino acid identity 87.7 and 86%, respectively. Inactivation of norA _SEP increased the ciprofloxacin susceptibility of E. coli DH5α carrying the pB SK 198 norA _SEP EZ cat norA _SEP plasmid.

Keywords: NorA-like gene; S. epidermidis; S. haemolyticus; coagulase negative staphylococci; efflux pumps.