Abstract
Covering: 2012 to 2019HemN-like radical S-adenosyl-l-methionine (SAM) enzymes have been recently disclosed to catalyze diverse chemically challenging reactions from primary to secondary metabolic pathways. In this highlight, we summarize the reaction examples catalyzed by HemN-like enzymes to date and the enzymatic mechanisms reported. From the recent mechanistic investigations, we reason that there is a shared initiating mechanism wherein a characteristic SAM methylene radical is proposed to abstract a hydrogen atom from an sp3 carbon or add onto an sp2 carbon center although variations occur thereafter from reaction to reaction, as well as providing a brief insight into some future prospects.
Publication types
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Bacterial Proteins / chemistry
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Bacterial Proteins / metabolism
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Coproporphyrinogen Oxidase / chemistry
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Coproporphyrinogen Oxidase / metabolism
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Duocarmycins / metabolism
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Enzymes / chemistry*
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Enzymes / metabolism*
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Escherichia coli Proteins / metabolism
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Heme / metabolism
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Hydrogen
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Methylation
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Peptides, Cyclic / metabolism
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Polyketides / metabolism
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Protein Methyltransferases / metabolism
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S-Adenosylmethionine / metabolism*
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Thiazoles / metabolism
Substances
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Bacterial Proteins
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Duocarmycins
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Enzymes
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Escherichia coli Proteins
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Peptides, Cyclic
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Polyketides
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Thiazoles
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jawsamycin
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thiomuracin A
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yatakemycin
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Heme
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CC 1065
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S-Adenosylmethionine
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Hydrogen
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HemN protein, Bacteria
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Coproporphyrinogen Oxidase
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ChuW protein, E coli
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Protein Methyltransferases
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nosiheptide