Transcriptome analysis revealed bisphenol A and nonylphenol affect reproduction

Reprod Toxicol. 2019 Sep:88:39-47. doi: 10.1016/j.reprotox.2019.06.006. Epub 2019 Jul 6.

Abstract

Effects of endocrine disrupting chemicals (EDCs) on reproduction have not been fully explained comprehensively. In this study, we tried to validate the common effect of Bisphenol A (BPA) and Nonylphenol (NP) on the differentiation of embryonic stem (ES) cells and found that they modify the expression of germ cell specific genes. To elucidate functional significance on biological process, we performed Gene Ontology (GO)-based microarray analysis comparing with published GeneChip data of primordial germ cell development in vivo. Cluster analysis of gene expression profile revealed that EDC treatment and primordial germ cell (PGC) development shared characteristic cluster consists of GO terms related to "germ cell development" and "reproduction". In the GO term "reproduction", meiosis related genes showed high expression level by EDC exposure. These results suggest that BPA and NP affect not only some of the germ cell specific genes, but functionally interferes germ cell development and reproduction.

Keywords: BPA; Biological process; Gene set enrichment analysis; Microarray; NP; Stra8.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Benzhydryl Compounds / toxicity*
  • Cells, Cultured
  • Embryonic Stem Cells / drug effects*
  • Gene Expression Profiling*
  • Germ Cells / drug effects
  • Germ Cells / growth & development
  • Mice
  • Mice, Inbred ICR
  • Oligonucleotide Array Sequence Analysis
  • Phenols / toxicity*
  • Reproduction / drug effects

Substances

  • Benzhydryl Compounds
  • Phenols
  • nonylphenol
  • bisphenol A