Objectives: To enhance the productivity of foreign protein in culture cells using baculovirus expression system.
Results: A low concentration of MβCD, with the optimal application concentration of 0.25 mM and the appropriate preincubation time range from 10 to 120 min, can efficiently enhance expression levels in both the AcMNPV and BmNPV expression systems.
Conclusions: Preincubation with a low concentration MβCD enhance baculovirus infection and foreign protein expression productivity.
Keywords: AcMNPV; BmNPV; Expression; Methyl-β-cyclodextrin.