Evaluation of the Immunochromatographic NG-Test Carba 5 for Rapid Identification of Carbapenemase in Nonfermenters

Antimicrob Agents Chemother. 2019 Aug 23;63(9):e00968-19. doi: 10.1128/AAC.00968-19. Print 2019 Sep.

Abstract

The immunochromatographic assay NG-Test Carba 5 (NG-Biotech) was evaluated with a collection of 107 carbapenemase-producing nonfermenters (CP-NF) (55 Pseudomonas spp., 51 Acinetobacter spp., and 1 Achromobacter xylosoxidans isolate) and 61 carbapenemase-negative isolates. All KPC, VIM, and NDM carbapenemase producers tested were accurately detected. Of the 16 IMP variants tested, 6 (37.5%) variants were not detected. Considering the epidemiology of CP-NFs in France, the NG-Test Carba 5 would detect 89.4% of CP Pseudomonas spp. but only 12.9% of CP Acinetobacter spp.

Keywords: Acinetobacter; IMP; KPC; NDM; Pseudomonas aeruginosa; VIM; carbapenemases; rapid diagnostic.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Achromobacter denitrificans / drug effects
  • Achromobacter denitrificans / enzymology
  • Achromobacter denitrificans / genetics*
  • Achromobacter denitrificans / isolation & purification
  • Acinetobacter / drug effects
  • Acinetobacter / enzymology
  • Acinetobacter / genetics*
  • Acinetobacter / isolation & purification
  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / genetics*
  • Carbapenems / pharmacology
  • Chromatography, Affinity / methods*
  • Chromatography, Affinity / standards
  • France / epidemiology
  • Gene Expression
  • Gram-Negative Bacterial Infections / drug therapy
  • Gram-Negative Bacterial Infections / epidemiology
  • Gram-Negative Bacterial Infections / microbiology
  • Humans
  • Microbial Sensitivity Tests / standards
  • Pseudomonas / drug effects
  • Pseudomonas / enzymology
  • Pseudomonas / genetics*
  • Pseudomonas / isolation & purification
  • Sensitivity and Specificity
  • Species Specificity
  • beta-Lactam Resistance / genetics*
  • beta-Lactamases / genetics*

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Carbapenems
  • beta-Lactamases
  • carbapenemase