Anti-tumor potential of cell free culture supernatant of Lactobacillus rhamnosus strains isolated from human breast milk

Muhammad Shahid Riaz Rajoka; Haobin Zhao; Hafiza Mahreen Mehwish; Na Li; Yao Lu; Ziyang Lian; Dongyan Shao; Mingliang Jin; Qi Li; Liqing Zhao; Junling Shi

doi:10.1016/j.foodres.2019.05.002

Anti-tumor potential of cell free culture supernatant of Lactobacillus rhamnosus strains isolated from human breast milk

Food Res Int. 2019 Sep:123:286-297. doi: 10.1016/j.foodres.2019.05.002. Epub 2019 May 3.

Authors

Affiliations

¹ Key Laboratory for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's Republic of China; Department of Food Science and Engineering, College of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen, Guangdong, People's Republic of China.
² Key Laboratory for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's Republic of China.
³ School of Pharmaceutical Sciences, Guangdong Key Laboratory for Genome Stability & Human Disease Prevention, Shenzhen Key Laboratory of Novel Natural Health Care Products, Engineering Laboratory of Shenzhen Natural small molecule Innovative Drugs, Health Science Center, Shenzhen University, Shenzhen 518060, PR China.
⁴ Department of Food Science and Engineering, College of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen, Guangdong, People's Republic of China.
⁵ Key Laboratory for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's Republic of China. Electronic address: sjlshi2004@nwpu.edu.cn.

PMID: 31284979
DOI: 10.1016/j.foodres.2019.05.002

Abstract

Lactobacilli rhamnosus has been characterized as a probiotic and plays an important role in human health by stimulating the supplement of nutrients, preventing the colonization of pathogens, and influencing the immune system. This study investigated the anticancer activity of the three Lactobacillus rhamnosus strains SHA111, SHA112, and SHA113 isolated from human breast milk. The cell-free supernatant of a liquid culture of the three strains showed excellent antioxidant activities against DPPH free radicals, superoxide anion radicals, and hydroxyl radicals; furthermore, significant anticancer activity was found on cervix cancer cells (HeLa) via cytotoxicity and induction of apoptosis. RT-qPCR and western blot analysis showed the induction of apoptosis was achieved via the up-regulation of BAD, BAX, Caspase3, Caspase8, Caspase9, and down-regulation of BCL-2 genes in HeLa cells. The results suggest that these strains have potential anticancer capability.

Keywords: Antioxidant; Antitumor; Apoptosis; Lactobacillus; Probiotics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antioxidants / metabolism
Apoptosis / physiology
Caspase 3 / genetics
Caspase 3 / metabolism
Caspase 8 / genetics
Caspase 8 / metabolism
Caspase 9 / genetics
Caspase 9 / metabolism
Cell Proliferation / physiology
Food Microbiology*
Gene Expression Regulation
HeLa Cells
Humans
Hydrogen Peroxide / metabolism
Lacticaseibacillus rhamnosus / classification
Lacticaseibacillus rhamnosus / isolation & purification*
Lacticaseibacillus rhamnosus / metabolism*
Membrane Potential, Mitochondrial
Milk, Human / microbiology*
Probiotics
Proto-Oncogene Proteins c-bcl-2 / genetics
Proto-Oncogene Proteins c-bcl-2 / metabolism
Reactive Oxygen Species / metabolism
bcl-2-Associated X Protein / genetics
bcl-2-Associated X Protein / metabolism
bcl-Associated Death Protein / genetics
bcl-Associated Death Protein / metabolism

Substances

Antioxidants
BAD protein, human
BAX protein, human
BCL2 protein, human
Proto-Oncogene Proteins c-bcl-2
Reactive Oxygen Species
bcl-2-Associated X Protein
bcl-Associated Death Protein
Hydrogen Peroxide
CASP3 protein, human
CASP8 protein, human
CASP9 protein, human
Caspase 3
Caspase 8
Caspase 9