The role of ERp44 in glucose and lipid metabolism

Arch Biochem Biophys. 2019 Aug 15:671:175-184. doi: 10.1016/j.abb.2019.06.011. Epub 2019 Jul 5.

Abstract

Endoplasmic Reticulum Protein 44 (ERp44) is a member of the PDI family, named for a molecular weight of 44 kD. White adipose tissue has metabolic and endocrine functions that are important to metabolism. The role of ERp44 in glucose and lipid metabolism is not known yet. The current study was undertaken to investigate the implication of ERp44 in glucose and lipid metabolism. In this study, we generated and characterized ERp44-/- mice. We used type 2 diabetes models and ERp44 knockout mice to show the implication of ERp44 in glucose and lipid metabolism. Knockout newborns had lower blood glucose compared to wild-type. Adult knockouts had abnormal intraperitoneal, glucose, insulin and pyruvic acid tolerance. Lipocytes were smaller and fewer in knockout mice compared to wild-type. Knockouts resisted to high-fat diet-induced obesity. ERp44 expression in white adipose tissue decreased significantly in type 2 diabetes models. Results suggest that ERp44 is closely associated with glucose and lipid metabolism.

Keywords: ERp44; Glucose/lipid metabolism disorder; Knockout mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue, White / metabolism
  • Adipose Tissue, White / pathology
  • Animals
  • Cell Line
  • Diabetes Mellitus, Experimental / metabolism*
  • Diet, High-Fat
  • Female
  • Gene Knockout Techniques
  • Islets of Langerhans / pathology
  • Lipid Droplets / pathology
  • Lipid Metabolism / physiology*
  • Male
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Molecular Chaperones / genetics
  • Molecular Chaperones / metabolism*
  • Obesity / metabolism
  • Rats, Wistar

Substances

  • ERp44 protein, mouse
  • Membrane Proteins
  • Molecular Chaperones