Identification of microRNAs responding to cold stress in Dongxiang common wild rice

Genome. 2019 Sep;62(9):635-642. doi: 10.1139/gen-2019-0015. Epub 2019 Jul 8.

Abstract

Low temperature is a vital effector of rice at different growth stages. MicroRNAs (miRNAs) play important roles in responding to abiotic and biotic stresses. Here, we confirmed the cold tolerance of Dongxiang common wild rice and explored the miRNAs differentially expressed under cold stress using genome-wide small RNA sequencing. In total, 16 miRNAs, nine upregulated and seven downregulated by cold stress, were characterized in Dongxiang common wild rice, and their target genes were predicted. Additionally, an AgriGO analysis of the target genes revealed that they were enriched in several terms related to cold-stress tolerance, suggesting a complex response mechanism, involving miRNAs, to cold stress in Dongxiang common wild rice.

Keywords: Dongxiang common wild rice; low temperature stress; microARN; microRNA; riz commun Dongxiang; stress de froid; séquençage génomique complet; whole genome-wide sequencing.

MeSH terms

  • Acclimatization / genetics
  • Cold-Shock Response / genetics
  • MicroRNAs / metabolism*
  • Oryza / genetics*
  • Oryza / physiology
  • RNA, Plant / metabolism*
  • Sequence Analysis, RNA

Substances

  • MicroRNAs
  • RNA, Plant