Paper-based point-of-care test with xeno nucleic acid probes

Jie Hu; Kang Xiao; Birui Jin; Xuyang Zheng; Fanpu Ji; Dan Bai

doi:10.1002/bit.27106

Paper-based point-of-care test with xeno nucleic acid probes

Biotechnol Bioeng. 2019 Oct;116(10):2764-2777. doi: 10.1002/bit.27106. Epub 2019 Jul 24.

Authors

Jie Hu¹, Kang Xiao^{2

3}, Birui Jin⁴, Xuyang Zheng⁵, Fanpu Ji⁶, Dan Bai^{7

8}

Affiliations

¹ Singapore Institute of Manufacturing Technology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.
² Chinese Center for Disease Control and Prevention, National Institute for Viral Disease Control and Prevention, Beijing, P. R. China.
³ Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou, P. R. China.
⁴ School of Materials and Chemical Engineering, Xi'an Technological University, Xi'an, P. R. China.
⁵ Department of Infectious Diseases, Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, P. R. China.
⁶ Department of Infectious Diseases, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, P. R. China.
⁷ Xi'an Institute of Flexible Electronics (IFE) & Xi'an Key Laboratory of Flexible Electronics (KLoFE), MIIT Key Laboratory of Flexible Electronics (KLoFE), Shaanxi Key Laboratory of Flexible Electronics (KLoFE), Northwestern Polytechnical University (NPU), Xi'an, Shaanxi, P. R. China.
⁸ Xi'an Institute of Biomedical Materials and Engineering (IBME) & Xi'an Key Laboratory of Biomedical Materials and Engineering (KLBME), Northwestern Polytechnical University (NPU), Xi'an, Shaanxi, P. R. China.

PMID: 31282991
DOI: 10.1002/bit.27106

Abstract

Bridging the unmet need of efficient point-of-care testing (POCT) in biomedical engineering research and practice with the emerging development in artificial synthetic xeno nucleic acids (XNAs), this review summarized the recent development in paper-based POCT using XNAs as sensing probes. Alongside the signal transducing mode and immobilization methods of XNA probes, a detailed evaluation of probe performance was disclosed. With these new aspects, both researchers in synthetic chemistry / biomedical engineering and physicians in clinical practice could gain new insights in designing, manufacturing and choosing suitable reagents and techniques for POCT.

Keywords: aptamer; chemical modification; paper-based analytical devices (PADs); point-of-care testing (POCT); xeno nucleic acids (XNAs).

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Humans
Nucleic Acid Probes / chemistry*
Point-of-Care Systems*
Point-of-Care Testing*

Substances

Nucleic Acid Probes