Direct genotyping from whole blood using alkaline polyethylene glycol

Xiaonan Liu; Chao Zhang; Kai Hua; Jianping Liang; Hang Li; Ting Ma; Juanli Zhu; Yali Cui

doi:10.1016/j.ab.2019.113351

Direct genotyping from whole blood using alkaline polyethylene glycol

Anal Biochem. 2019 Oct 1:582:113351. doi: 10.1016/j.ab.2019.113351. Epub 2019 Jul 3.

Authors

Xiaonan Liu¹, Chao Zhang¹, Kai Hua², Jianping Liang¹, Hang Li¹, Ting Ma³, Juanli Zhu⁴, Yali Cui⁵

Affiliations

¹ College of Life Sciences, Northwest University, Xi'an, Shaanxi, 710069, China.
² College of Life Sciences, Northwest University, Xi'an, Shaanxi, 710069, China; Shaanxi Provincial Engineering Research Center for Nano-Biomedical Detection, Xi'an, Shaanxi, 710077, China.
³ Shaanxi Provincial People's Hospital, Xi'an, 710068, China.
⁴ Shaanxi Provincial Engineering Research Center for Nano-Biomedical Detection, Xi'an, Shaanxi, 710077, China.
⁵ College of Life Sciences, Northwest University, Xi'an, Shaanxi, 710069, China; Shaanxi Provincial Engineering Research Center for Nano-Biomedical Detection, Xi'an, Shaanxi, 710077, China. Electronic address: yalicui@nwu.edu.cn.

PMID: 31278897
DOI: 10.1016/j.ab.2019.113351

Abstract

In order to simplify biological sample preparation to meet the demand of rapid genotyping, we improve alkaline polyethylene glycol (APEG) based on Chomczynski's procedure for the PCR-based lateral flow genotyping system, which enables the rapid and efficient direct genotyping from whole blood without DNA purification. The improved APEG has a high tolerance for extreme storage conditions. Testing whole blood with an abnormal hematological index indicates that APEG efficiency would not be influenced by pathological factors. Compared with sequencing, the accuracy of this genotyping system was 100% on testing with 200 clinical samples.

Keywords: Alkaline polyethylene glycol; Direct genotyping; Whole blood.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Blood*
DNA / genetics
Genotype
Genotyping Techniques / methods*
Healthy Volunteers
Humans
Polyethylene Glycols / chemistry
Polymerase Chain Reaction / methods

Substances

Polyethylene Glycols
DNA