A nitroxyl-responsive near-infrared fluorescent chemosensor for visualizing H2S/NO crosstalk in biological systems

Mingwang Yang; Jiangli Fan; Wen Sun; Jianjun Du; Saran Long; Kun Shao; Xiaojun Peng

doi:10.1039/c9cc04060a

A nitroxyl-responsive near-infrared fluorescent chemosensor for visualizing H₂S/NO crosstalk in biological systems

Chem Commun (Camb). 2019 Jul 18;55(59):8583-8586. doi: 10.1039/c9cc04060a.

Authors

Mingwang Yang¹, Jiangli Fan², Wen Sun², Jianjun Du², Saran Long², Kun Shao², Xiaojun Peng²

Affiliations

¹ State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, Dalian, 116024, P. R. China.
² State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, Dalian, 116024, P. R. China and Shenzhen Research Institute, Dalian University of Technology, Gaoxin South fourth Road, Nanshan District, Shenzhen 518057, P. R. China. fanjl@dlut.edu.cn.

PMID: 31274135
DOI: 10.1039/c9cc04060a

Abstract

We present a near-infrared (NIR) fluorescent probe, NR-HNO, which was successfully applied to visualizing H2S/NO "crosstalk" by the fluorescence detection of nitroxyl with a fast response time (5 min) and a large Stokes shift (131 nm) in living cells and tissue; it was also used to image nitroxyl in live mice.

MeSH terms

Animals
Benzylidene Compounds / chemistry*
Benzylidene Compounds / radiation effects
Cell Line, Tumor
Fluorescent Dyes / chemistry*
Fluorescent Dyes / radiation effects
Humans
Hydrogen Sulfide / metabolism*
Kidney / metabolism
Light
Limit of Detection
Liver / metabolism
Mice
Microscopy, Fluorescence / methods
Nitric Oxide / metabolism*
Nitrites / chemistry
Nitrogen Oxides / analysis*
Nitrogen Oxides / metabolism
Spectrometry, Fluorescence / methods

Substances

Benzylidene Compounds
Fluorescent Dyes
Nitrites
Nitrogen Oxides
oxyhyponitrite
Nitric Oxide
nitroxyl
Hydrogen Sulfide