Cells are the basic units of life, and can be mimicked to create artificial analogs enabling the investigation of cellular mechanisms under controlled conditions. Building biomimetic systems ranging from proto-cells to cell-like objects such as compartment membranes can be achieved by collecting biobricks that self-assemble to build simplified models performing specific functions. Hence, scientists can develop and optimize new synthetic cells with biological functions by taking inspiration from nature and exploiting the advantages of synthetic biology. However, the bottom-down approach is not restricted to the basic principles of biological cells, and new mimicry systems can be designed starting with a combination of living and non-living simple molecules to focus on a cellular machinery function. In recent years, microfluidic devices have been well established to engineer bioarchitecture models resembling cell-like structures involving vesicles, compartmentalization, synthetic membranes, and the chip itself as a synthetic cell. This review aims to highlight the role of biological cells and their impact on inspiring the development of biomimetic models. The combination of the principles of synthetic biology with microfluidic technology represents the newly-introduced field of synthetic cells and synthetic membranes that can be further exploited in diagnostic and therapeutic applications.
Keywords: Microfluidic technology; Protocells; Synthetic bioarchitectures; Synthetic biology.