Niacinamide Protects Skin Cells from Oxidative Stress Induced by Particulate Matter

Ao Xuan Zhen; Mei Jing Piao; Kyoung Ah Kang; Pincha Devage Sameera Madushan Fernando; Hee Kyoung Kang; Young Sang Koh; Joo Mi Yi; Jin Won Hyun

doi:10.4062/biomolther.2019.061

Niacinamide Protects Skin Cells from Oxidative Stress Induced by Particulate Matter

Biomol Ther (Seoul). 2019 Nov 1;27(6):562-569. doi: 10.4062/biomolther.2019.061.

Authors

Ao Xuan Zhen¹, Mei Jing Piao¹, Kyoung Ah Kang¹, Pincha Devage Sameera Madushan Fernando¹, Hee Kyoung Kang¹, Young Sang Koh¹, Joo Mi Yi², Jin Won Hyun¹

Affiliations

¹ Jeju National University School of Medicine and Jeju Research Center for Natural Medicine, Jeju 63243, Republic of Korea.
² Department of Microbiology and Immunology, Inje University College of Medicine, Busan 47392, Republic of Korea.

Abstract

Niacinamide (NIA) is a water-soluble vitamin that is widely used in the treatment of skin diseases. Moreover, NIA displays antioxidant effects and helps repair damaged DNA. Recent studies showed that particulate matter 2.5 (PM_2.5) induced reactive oxygen species (ROS), causing disruption of DNA, lipids, and proteins; mitochondrial depolarization, and apoptosis of skin keratinocytes. Here, we investigated the protective effects of NIA on PM_2.5-induced oxidative stress in human HaCaT keratinocytes. We found that NIA could inhibit the ROS generation induced by PM_2.5, as well blocked the PM_2.5-induced oxidation of molecules, such as lipids, proteins, and DNA. Furthermore, NIA alleviated PM_2.5-induced accumulation of cellular Ca²⁺, which caused cell membrane depolarization and apoptosis, and reduced the number of apoptotic cells. Collectively, the findings show that NIA can protect keratinocytes from PM_2.5-induced oxidative stress and cell damage.

Keywords: Human HaCaT keratinocyte; Niacinamide; Oxidative stress; Particulate matter 2.5.