Collection of Recombinant Rotaviruses Expressing Fluorescent Reporter Proteins

Asha A Philip; Brittany E Herrin; Maximiliano L Garcia; Andrew T Abad; Sarah P Katen; John T Patton

doi:10.1128/MRA.00523-19

Collection of Recombinant Rotaviruses Expressing Fluorescent Reporter Proteins

Microbiol Resour Announc. 2019 Jul 3;8(27):e00523-19. doi: 10.1128/MRA.00523-19.

Authors

Asha A Philip¹, Brittany E Herrin¹, Maximiliano L Garcia¹, Andrew T Abad¹, Sarah P Katen¹, John T Patton²

Affiliations

¹ Department of Biology, Indiana University, Bloomington, Indiana, USA.
² Department of Biology, Indiana University, Bloomington, Indiana, USA jtpatton@iu.edu.

Abstract

A collection of recombinant rotaviruses that express the fluorescent markers UnaG, mKate, mRuby, TagBFP, CFP, or YFP as separate proteins was generated. Genes for the fluorescent proteins were inserted into genome segment 7 without compromising expression of the protein NSP3. These recombinant rotaviruses are valuable for analyzing rotavirus biology by fluorescence-based live-cell imaging.

Abstract

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