Enhancement of disease resistance, growth potential, and photosynthesis in tomato (Solanum lycopersicum) by inoculation with an endophytic actinobacterium, Streptomyces thermocarboxydus strain BPSAC147

Ajit Kumar Passari; Kalidas Upadhyaya; Garima Singh; Ahmed M Abdel-Azeem; Sugitha Thankappan; Sivakumar Uthandi; Abeer Hashem; Elsayed Fathi Abd Allah; Jahangir Ahmed Malik; Alqarawi As; Vijai Kumar Gupta; Sanjay Ranjan; Bhim Pratap Singh

doi:10.1371/journal.pone.0219014

Enhancement of disease resistance, growth potential, and photosynthesis in tomato (Solanum lycopersicum) by inoculation with an endophytic actinobacterium, Streptomyces thermocarboxydus strain BPSAC147

PLoS One. 2019 Jul 3;14(7):e0219014. doi: 10.1371/journal.pone.0219014. eCollection 2019.

Authors

Affiliations

¹ Department of Biotechnology, Mizoram University, Aizawl, Mizoram, India.
² Departamento de Biologia Molecular y Biotecnologia, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico (UNAM), Mexico, Mexico.
³ Department of Forestry, Mizoram University, Aizawl, Mizoram, India.
⁴ Department of Botany, Suez Canal University, Ismailia, Egypt.
⁵ Biocatalysts Lab, Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, India.
⁶ Botany and Microbiology Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
⁷ Mycology and Plant Disease Survey Department, Plant Pathology Research Institute, ARC, Giza, Egypt.
⁸ Department of Chemistry and Biotechnology, ERA Chair of Green Chemistry, Tallinn University of Technology, Tallinn, Estonia.
⁹ Plant Production Department, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia.
¹⁰ Application Scientist, Spectraritec, Ranjit Nagar Commercial Complex, Saadipur, Delhi.

Abstract

Biotic stresses in plants have a significant impact on agricultural productivity. In the present study, in vivo experiments were conducted to determine the physiological responses of tomato (Solanum lycopersicum L.) seedlings by inoculation with an endophytic actinobacterium, Streptomyces thermocarboxydus isolate BPSAC147 under greenhouse conditions. Further, photochemical quantum yield of photosystem II (PSII) (Fv/Fm), photochemical quenching (qP) and non-photochemical (NPQ) were calculated in seedlings inoculated with S. thermocarboxydus (T1) and were compared with control (T0) plants. Furthermore, the electron transport rate (ETR) of PSII exhibited a significant increase in T1 plants, relative to T0 plants. These results indicate that inoculation of tomato seedlings with S. thermocarboxydus had a positive effect on the process of photosynthesis, resulting in enhanced chlorophyll fluorescence parameters due to increased ETR in the thylakoid membrane. GC-MS analysis showed significant differences in the volatile compounds in the different treatments performed under greenhouse conditions. The present study suggests that S. thermocarboxydus can be used as new biocontrol agent to control Fusarium wilt in tomato crops and enhance productivity by enhancing photosynthesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biological Control Agents
Chlorophyll / metabolism
Disease Resistance
Electron Transport
Endophytes / classification
Endophytes / genetics
Endophytes / physiology*
Fusarium / pathogenicity
Germination
Photosynthesis
Phylogeny
Plant Diseases / microbiology
Plant Diseases / prevention & control
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics
Solanum lycopersicum / growth & development
Solanum lycopersicum / microbiology*
Solanum lycopersicum / physiology
Streptomyces / classification
Streptomyces / genetics
Streptomyces / physiology*

Substances

Biological Control Agents
RNA, Bacterial
RNA, Ribosomal, 16S
Chlorophyll

Supplementary concepts

Streptomyces thermocarboxydus

Grants and funding

BPS is thankful to the Department of Biotechnology, Government of India, New Delhi for financial support under DBT`s Unit of Excellence programme for NE (102/IFD/SAN/4290- 4291/2016-2017). The authors would like to extend their sincere appreciation to the Deanship of Scientific Research at King Saud University for its funding to the Research Group number (RG-1435-014). Author SR is employed by Spectraritec. Spectraritec provided support in the form of salary for author SR, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific role of this author is articulated in the ‘author contributions’ section.