Dual sensing of glutathione and acidic pH values by using MnO2 nanosheets and 3-acetyl-7-hydroxy-2H-chromen-2-one as a fluorescent pH probe

Lei Fu; Yanlin Du; Zhenxi Zhang; Haiyan Sun; Aixian Zheng; Xiaolong Liu

doi:10.1007/s00604-019-3590-7

Dual sensing of glutathione and acidic pH values by using MnO₂ nanosheets and 3-acetyl-7-hydroxy-2H-chromen-2-one as a fluorescent pH probe

Mikrochim Acta. 2019 Jul 2;186(8):491. doi: 10.1007/s00604-019-3590-7.

Authors

Lei Fu^#¹, Yanlin Du^#^{2

3}, Zhenxi Zhang¹, Haiyan Sun⁴, Aixian Zheng^{5

6}, Xiaolong Liu^{7

8

9}

Affiliations

¹ The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, People's Republic of China.
² The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, People's Republic of China.
³ College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.
⁴ Department of Anesthesiology, Beijing Anzhen Hospital, Capital Medical University, Beijing, 100029, People's Republic of China.
⁵ The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, People's Republic of China. zax040500273@126.com.
⁶ The Liver Center of Fujian Province, Fujian Medical University, Fuzhou, 350025, People's Republic of China. zax040500273@126.com.
⁷ The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, People's Republic of China. xiaoloong.liu@gmail.com.
⁸ The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, People's Republic of China. xiaoloong.liu@gmail.com.
⁹ The Liver Center of Fujian Province, Fujian Medical University, Fuzhou, 350025, People's Republic of China. xiaoloong.liu@gmail.com.

^# Contributed equally.

PMID: 31267269
DOI: 10.1007/s00604-019-3590-7

Abstract

A multifunctional nanoprobe is described for dual sensing of acidic pH values and glutathione (GSH) by combining the pH-responsive fluorescent probe 3-acetyl-7-hydroxy-2H-chromen-2-one (AHC) and MnO₂ nanosheets. The fluorescence of the MnO₂/AHC composite is weak due to an inner filter effect. If, however, the MnO₂ nanosheets are reductively decomposed by GSH, the blue fluorescence of the pH probe AHC (with excitation/emission maximum at 417/456 nm) will be restored. The MnO₂ nanosheets also are decomposed by acidic pH values, and the fluorescence of AHC is decreased. According to absorbance and fluorescence signal changes, the pH and GSH induced responses can be easily distinguished. Thus, the nanoprobe can be used for logical analysis of acidic pH values and GSH. The nanoprobe works in the pH range from 4 to 7, and GSH can be determined in the concentration range from 0.5 to 200 μM. Graphical abstract Schematic presentation of a multifunctional nanoprobe for dual sensing of acidic pH values and glutathione by combining a pH-responsive fluorescent probe and MnO₂ nanosheets. According to absorbance and fluorescence signal changes, the nanoprobe can be used for logical analysis.

Keywords: Inner filter effect; Intramolecular charge transfer; Logical analysis; Multifunctional nanoprobe; Phenol group; pH-responsive fluorescent dye.

Publication types

Research Support, Non-U.S. Gov't

Abstract

Publication types

Grants and funding