Evaluation of morphology, morphometry and follicular dynamics in FecGE genotyped ewes

Abstract

This study aimed to evaluate the effect of FecG^E mutation on the development of ovarian follicles. To this end, 42 Santa Inês ewes were genotyped for FecG^E mutation and classified as wild-type (FecG^+/+), heterozygous (FecG^+/E) or mutant homozygous (FecG^E/E). Ovarian fragments were processed, and the follicles were analyzed with regard to the morphology and morphometry using classical histology. For the evaluation of follicular dynamics, ewes underwent oestrous synchronization and were monitored throughout an interovulatory period. A higher (P < 0.05) percentage of morphologically normal follicles in the primordial stage was identified in FecG^E/E (90.0%) and FecG^+/E (88.1%) ewes than in the FecG^+/+ (73.0%) ewes. There was also a significantly greater (P < 0.05) number of morphologically normal follicles in the FecG^E/E (87.3%) and FecG^+/E (83.3%) ewes than in FecG^+/+ (76.8%) ewes in the transitional stage. A smaller (P < 0.05) diameter was observed in the secondary follicles in FecG^E/E (93.8 μm) ewes than in FecG^+/E (171.8 μm) ewes. Regarding follicular dynamics, FecG^E/E ewes showed a greater (P < 0.05) number of ovulations (2.5 ± 0.2) than FecG^+/+ ewes (1.5 ± 0.3) ewes. Ovulatory follicles were smaller (P < 0.05) in the FecG^E/E (5.1 mm) and FecG^+/E (5.2 mm) ewes than in FecG^+/+ (5.8 mm) ewes. Santa Inês nulliparous ewes carrying the FecG^E mutation showed a greater proportion of morphologically normal follicles in the primordial and transitional stages than those not carrying the mutation. FecG^E/E ewes demonstrated a higher number of ovulated follicles and that FecG^E/E and FecG^+/E ewes presented ovulatory follicles with a smaller diameter.

Keywords: GDF9; Ovine; Polymorphism; Prolificacy.