Updates on the Current Technologies for microRNA Profiling

Rebecca Mathew; Valentina Mattei; Muna Al Hashmi; Sara Tomei

doi:10.2174/2211536608666190628112722

Updates on the Current Technologies for microRNA Profiling

Microrna. 2020;9(1):17-24. doi: 10.2174/2211536608666190628112722.

Authors

Rebecca Mathew¹, Valentina Mattei¹, Muna Al Hashmi¹, Sara Tomei¹

Affiliation

¹ Omics Core and Biorepository, Sidra Medicine, Out Patient Clinic, Doha, Qatar.

PMID: 31264553
DOI: 10.2174/2211536608666190628112722

Abstract

MicroRNAs are RNA molecules of ~22 nt length that regulate gene expression posttranscriptionally. The role of miRNAs has been reported in many cellular processes including apoptosis, cell differentiation, development and proliferation. The dysregulated expression of miRNAs has been proposed as a biomarker for the diagnosis, onset and prognosis of human diseases. The utility of miRNA profiles to identify and discriminate patients from healthy individuals is highly dependent on the sensitivity and specificity of the technologies used for their detection and the quantity and quality of starting material. In this review, we present an update of the current technologies for the extraction, QC assessment and detection of miRNAs with special focus to the most recent methods, discussing their advantages as well as their shortcomings.

Keywords: Microarray; PCR; miRNA; miRNA extraction; quality control; sequencing..

Publication types

Review

MeSH terms

Gene Expression / genetics
Gene Expression Profiling / methods*
Gene Expression Regulation / genetics
High-Throughput Nucleotide Sequencing / methods*
Humans
MicroRNAs / genetics*
Oligonucleotide Array Sequence Analysis / methods*
Polymerase Chain Reaction / methods*
Quality Control

Substances

MicroRNAs