Advances in Imaging Plant Cell Walls

Yuanyuan Zhao; Yi Man; Jialong Wen; Yayu Guo; Jinxing Lin

doi:10.1016/j.tplants.2019.05.009

Advances in Imaging Plant Cell Walls

Trends Plant Sci. 2019 Sep;24(9):867-878. doi: 10.1016/j.tplants.2019.05.009. Epub 2019 Jun 27.

Authors

Yuanyuan Zhao¹, Yi Man¹, Jialong Wen², Yayu Guo¹, Jinxing Lin³

Affiliations

¹ Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China; College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
² Beijing Key laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.
³ Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China; College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. Electronic address: linjx@ibcas.ac.cn.

PMID: 31257154
DOI: 10.1016/j.tplants.2019.05.009

Abstract

Understanding of cell wall architecture, including the crosslinking of cell wall polymers, provides crucial information for elucidating the relationship between cell wall structure and cell function. Moreover, examination of the cell wall informs efforts to improve biomass breakdown in bioreactor conditions. Over the past decades, imaging techniques have been used extensively to reveal the structural organization and chemical composition of cell walls, but detailed imaging of the native composition and architecture of the cell wall remains challenging. Here, we review progress in the development of cell wall imaging techniques. In particular, we focus on several advanced, label-free techniques for imaging cell walls and their potential applications in investigation of the biological functions of plant cell walls.

Keywords: label-free imaging; microscopy; plant cell wall; stimulated Raman scattering (SRS).

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Biomass
Cell Membrane
Cell Wall*