Development of enhanced sensitivity protocols on the RapidHIT™ 200 with a view to processing casework material

David Shackleton; Jenny Pagram; Nicholas Andrews; Simon Malsom; Lesley Ives; Des Vanhinsbergh

doi:10.1016/j.scijus.2019.03.002

Development of enhanced sensitivity protocols on the RapidHIT™ 200 with a view to processing casework material

Sci Justice. 2019 Jul;59(4):411-417. doi: 10.1016/j.scijus.2019.03.002. Epub 2019 Mar 6.

Authors

David Shackleton¹, Jenny Pagram², Nicholas Andrews³, Simon Malsom³, Lesley Ives³, Des Vanhinsbergh³

Affiliations

¹ Key Forensic Services, 4 Penfold Drive, Wymondham, Norfolk NR18 0WZ, UK. Electronic address: david.shackleton@keyforensic.co.uk.
² Key Forensic Services, 207c Cavendish Place, Birchwood Park, Warrington, Cheshire WA3 6WU, UK.
³ Key Forensic Services, 4 Penfold Drive, Wymondham, Norfolk NR18 0WZ, UK.

PMID: 31256812
DOI: 10.1016/j.scijus.2019.03.002

Abstract

The RapidHIT™ 200 device from IntegenX® provides a sample-to-profile platform that is capable of processing a variety of sample types. In this study we review the sensitivity of the 'Run Other' protocol for processing crime stain type samples containing various input quantities of DNA using the AmpFℓSTR® NGMSElect™ Express PCR Amplification Kit cartridges available from IntegenX®. The range of DNA inputs which achieved useable results were not as desired and therefore various enhancements to the instruments extraction processes were investigated. These studies showed an improvement in the range of DNA input templates that could by processed on the RapidHIT™ 200 by using the enhanced methods and resulted in three new run protocols.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Alleles
Blood Stains
Cell Line
DNA Fingerprinting / instrumentation*
DNA Fingerprinting / methods*
Humans
Male
Real-Time Polymerase Chain Reaction
Templates, Genetic*