Stabilization of microtubules by cevipabulin

Syeda Rubaiya Nasrin; Arif Md Rashedul Kabir; Akihiko Konagaya; Tsukasa Ishihara; Kazuki Sada; Akira Kakugo

doi:10.1016/j.bbrc.2019.06.095

Stabilization of microtubules by cevipabulin

Biochem Biophys Res Commun. 2019 Aug 27;516(3):760-764. doi: 10.1016/j.bbrc.2019.06.095. Epub 2019 Jun 26.

Authors

Syeda Rubaiya Nasrin¹, Arif Md Rashedul Kabir², Akihiko Konagaya³, Tsukasa Ishihara⁴, Kazuki Sada⁵, Akira Kakugo⁶

Affiliations

¹ Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo, 060-0810, Hokkaido, Japan.
² Faculty of Science, Hokkaido University, Sapporo, 060-0810, Hokkaido, Japan.
³ Department of Computational Intelligence and Systems Science, Tokyo Institute of Technology, Yokohama, 226-8502, Japan.
⁴ Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, 305-8566, Japan.
⁵ Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo, 060-0810, Hokkaido, Japan; Faculty of Science, Hokkaido University, Sapporo, 060-0810, Hokkaido, Japan.
⁶ Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo, 060-0810, Hokkaido, Japan; Faculty of Science, Hokkaido University, Sapporo, 060-0810, Hokkaido, Japan. Electronic address: kakugo@sci.hokudai.ac.jp.

PMID: 31253401
DOI: 10.1016/j.bbrc.2019.06.095

Abstract

We report the utility of cevipabulin as a stabilizing agent for microtubules. Cevipabulin-stabilized microtubules were more flexible compared to the microtubules stabilized by paclitaxel, the most commonly used microtubule stabilizing agent. Similar to the paclitaxel-stabilized microtubules, cevipabulin-stabilized microtubules were driven by kinesins in an in vitro gliding assay. The velocity of cevipabulin-stabilized microtubules was significantly higher than that of paclitaxel-stabilized microtubules. These findings will enrich the variety of microtubules with difference in mechanical and dynamic properties and widen their applications in nanotechnology.

Keywords: Cevipabulin; Kinesin; Microtubule; Motility assay; Persistence length.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Hydrocarbons, Halogenated / chemistry
Hydrocarbons, Halogenated / metabolism*
Hydrocarbons, Halogenated / pharmacology
Microscopy, Fluorescence
Microtubules / metabolism*
Molecular Docking Simulation*
Molecular Structure
Nanotechnology
Paclitaxel / chemistry
Paclitaxel / metabolism
Paclitaxel / pharmacology
Protein Stability / drug effects
Swine
Time-Lapse Imaging / methods
Triazoles / chemistry
Triazoles / metabolism*
Triazoles / pharmacology
Tubulin / metabolism*

Substances

Hydrocarbons, Halogenated
Triazoles
Tubulin
cevipabulin
Paclitaxel