Compositional difference in infant formula (IF) tends to influence its functionality and storage behaviour. The aim was to study the composition and physico-chemical properties of different stages of two commercial IF (A and B). Lactose crystallization measured by X-ray diffraction ranged between 2 and 32 % and was observed to decrease with increasing IF stages, which directly correlates with their composition. Scanning electron microscopy confirmed the presence of crystalline lactose which significantly (p < 0.05) increased the powder particle size. On the contrary, a negative correlation was observed between surface fat and lactose crystallization in all samples. Bulk and surface-free fat composition was significantly (p < 0.05) different for all samples. Surface free-fat analysis showed restricted presence (5-10% of surface fat) of unsaturated fatty acids (C18:1 and C18:2) in IF with higher crystalline lactose as opposed to >40% in others, suggesting a possible role of lactose crystallization in preferential migration of triglycerides to particle surface.
Keywords: Infant formula; Lactose; Powder particle; Surface free fat.
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