The aim of this study was to evaluate the effects of the order of grass silage (GS) and maize silage (MS) supplementation on milk yield, grazing behavior and nitrogen (N) partitioning of lactating dairy cows during autumn. Thirty-six Holstein-Friesian dairy cows were randomly assigned to one of three treatments, and cows remained on these treatments for a 62 days period: (1) MIX; cows supplemented with 3 kg of dry matter (DM) of silage containing 1.5 kg DM of MS and 1.5 kg DM of GS in both the morning and afternoon; (2) GS-MS; cows supplemented with 3 kg DM of GS in the morning and 3 kg DM of MS in the afternoon; (3) MS-GS; cows supplemented with 3 kg DM of MS in the morning and 3 kg DM of GS in the afternoon. All cows received a pasture allowance of 17 kg DM/cow/d and 3 kg DM of concentrate. Grazing time and pasture intake were unaffected by treatment; however, milk production was greater for MS-GS, while milk protein was greater for GS-MS. Urinary N excretion was greater for MS-GS than MIX. In conclusion, MS-GS resulted in high milk yield but also high urinary N excretion, while MIX resulted in low urinary N excretion but also decreased milk yield.
Keywords: grazing behavior; milk yield; nitrogen excretion; rumen function; supplement allocation.