Weekly Variation of Rotavirus A Concentrations in Sewage and Oysters in Japan, 2014-2016

Erika Ito; Jian Pu; Takayuki Miura; Shinobu Kazama; Masateru Nishiyama; Hiroaki Ito; Yoshimitsu Konta; Gia Thanh Nguyen; Tatsuo Omura; Toru Watanabe

doi:10.3390/pathogens8030089

Weekly Variation of Rotavirus A Concentrations in Sewage and Oysters in Japan, 2014-2016

Pathogens. 2019 Jun 26;8(3):89. doi: 10.3390/pathogens8030089.

Authors

Erika Ito¹, Jian Pu², Takayuki Miura³, Shinobu Kazama⁴, Masateru Nishiyama⁵, Hiroaki Ito⁶, Yoshimitsu Konta⁷, Gia Thanh Nguyen⁸, Tatsuo Omura⁷, Toru Watanabe⁵

Affiliations

¹ The United Graduate School of Agricultural Sciences, Iwate University, Iwate 020-8550, Japan.
² Faculty of Information Networking for Innovation and Design, Toyo University, Tokyo 115-0053, Japan. pu@toyo.jp.
³ Department of Environmental Health, National Institute of Public Health, Saitama 351-0197, Japan.
⁴ Department of Urban Engineering, The University of Tokyo, Tokyo 113-8656, Japan.
⁵ Department of Food, Life and Environmental Sciences, Yamagata University, Yamagata 997-8555, Japan.
⁶ Center for Water Cycle, Marine Environment and Disaster Management, Kumamoto University, Kumamoto 860-8555, Japan.
⁷ New Industry Creation Hatchery Center, Tohoku University, Miyagi 980-8579, Japan.
⁸ Department of Environmental and Occupational Health, College of Medicine and Pharmacy, Hue University, Hue city 530000, Vietnam.

Abstract

Concentrations of rotavirus A, in sewage and oysters collected weekly from September 2014 to April 2016 in Japan, were investigated using RT-qPCR; results showed up to 6.5 log₁₀ copies/mL and 4.3 log₁₀ copies/g of digestive tissue (DT) in sewage and oysters, respectively. No correlation was found between rotavirus concentration in sewage and oysters and cases of rotavirus-associated gastroenteritis.

Keywords: oyster; real-time PCR; rotavirus; sewage.

Grants and funding

CREST "Innovation of water monitoring system with rapid, highly precise and exhaustive pathogen detection technologies"/Japan Science and Technology Agency